For this reason, many peer-reviewed journals require or recommend the use of unbiased stereology when quantifying cells or structures. View a powerpoint presentation on 'Stereology Therory and Experimental Design'īy using unbiased stereology, researchers get unbiased, accurate and reliable data. Other techniques for obtaining quantitative analyses of cell populations, such as counting cell profiles at the top of each physical section, or using ad hoc automatic cell detection algorithms, yield inaccurate and biased results. Then, researchers use a set of rigorously tested rules to mark cells and structures in each sampling site for quantification.
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Unbiased stereology uses systematic random sampling (a statistical sampling method) to unbiasedly select a representative sample of the region of interest.
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Given stationarity with respect to time, absence of image artefacts and appropriate definition of chamber boundaries, for each time frame unbiased estimates of total blood volume in the relevant heart chambers were efficiently obtained using the Cavalieri method and point counting, Combined with a proper MRI acquisition, modern stereological methods constitute an efficient and reliable tool to quantify cardiac function noninvasively.Unbiased stereology is recognized in biological research as the best-practice method for quantitative histology. Given stationarity with respect to time, absence of image artefacts and appropriate definition of chamber boundaries, for each time frame unbiased estimates of total blood volume in the relevant heart chambers were efficiently obtained using the Cavalieri method and point counting, Combined with a proper MRI acquisition, modern stereological methods constitute an efficient and reliable tool to quantify cardiac function noninvasively.ĪB - Design-based stereology and phase contrast magnetic resonance imaging (MRI) were combined to monitor changes in the volume of the four chambers of the human heart during the cardiac cycle, The data set consisted of 18 adjacent slices (or 'scanning levels') of 0.5 cm thickness, perpendicular to the long axis of the body, and encompassing the whole heart of a healthy volunteer, At each scanning level, a cardiac gated MR image was obtained at each of 16 equally spaced time frames within the cardiac cycle. N2 - Design-based stereology and phase contrast magnetic resonance imaging (MRI) were combined to monitor changes in the volume of the four chambers of the human heart during the cardiac cycle, The data set consisted of 18 adjacent slices (or 'scanning levels') of 0.5 cm thickness, perpendicular to the long axis of the body, and encompassing the whole heart of a healthy volunteer, At each scanning level, a cardiac gated MR image was obtained at each of 16 equally spaced time frames within the cardiac cycle. T1 - Analysis of cardiac function by MRI and stereology